Chiaki Yoshikawa1*, Takashi Hoshiba2, Keita Sakakibara3, Yoshinobu Tsujii3


1WPI-MANA, National Institute for Materials Science 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047 Japan, 2Graduate School of Science and Engineering, Yamagata University
4-3-16 Jonan, Yonezawa, Yamagata 992-8510 Japan, 3Institute for Chemical Research, Kyoto University Uji, Kyoto 611-0011 Japan


Recently, we have developed cellulose nanofibers (CNFs) with concentrated polymer brushes (CPBs). One of the most potential applications of the CNF-CPB is believed to be used as a tissue engineering scaffold because it has unique physical properties as well as biocompatibility1. In this work, in order to develop a new CPB-based scaffold, we investigated cell behaviour against the CNF-CPB.

The initiating sites for atom transfer radical polymerization (ATRP) were introduced on the CNF surface by esterification as shown in figure 1. Polymer brushes with different graft densities (in CPB and semi-dilute polymer brush (SDPB) regimes) were prepared on the CNFs by ATRP. Then cells were mixed with the CNFs, and the mixtures were placed in a 5% CO2 and 37°C incubator for the prescribed time. Cells aggregated with the CNF-CPB, while none with the CNF-SDPB. We observed cell adhesion by a confocal microscope and investigated cell functions by gene expression analysis. The details will be discussed



[1] Lin, N and Dufresne, A. Eur. Polym. Jour. 2014, 59, 302-325. Nanocellulose in biomdecine: Current Status and Future Perspective.

Biographic Details

Dr. Chiaki Yoshikawa

Senior Researcher

WPI-MANA, National Institute for Materiasl Science, Japan:

Phone: +81 (0)29 860 4717 E-mail:

Research interests: Polymer brush, biointerface, biomaterials


Hawken N201