Waleed Hassanain, Emad Kiriakous*, Michael Schmidt, Godwin Ayoko


2 George Street
Queensland University of Technology
Brisbane 4001, QLD, Australia


Rapid, selective and sensitive label free surface enhanced Raman spectroscopy (SERS) method has been developed for the combined selective isolation and determination of environmental toxin Microcystin-LR (MC-LR) in biological samples. Smart magnetic core gold nanoparticles functionalized with MC-LR antibody fragments were used to selectively extract MC-LR from water and horse plasma matrices within 15 minutes. The purified MC-LR was later released by changing the pH of the nanoparticles without compromising the activity of the antibody fragments. Therefore, the regenerated nanosensor can be used again to re-capture MC-LR from multiple samples. Gold coated silicon nanopillars and paper SERS substrates were used for the screening of the released purified toxin with handheld Raman spectrometer. The limit of quantification on the nanopillars substrate was 10 fM (R2=0.9975) which satisfies the WHO cut off limit of 1 nM. The SERS results were qualitatively and quantitatively cross validated against ELISA technique. The new method showed strong potential for the rapid, sensitive and cost effective screening of other environmental toxins, peptides and proteins biomarkers in environmental monitoring and biomedical applications.


Biographic Details

Name: Waleed Hassanain

Title: PhD student

Affiliation, Country: School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Australia

Phone: +610470344424, E-mail: w.hassanain@qut.edu.au

Research interests: Analytical Chemistry, Nanomaterials, SERS, Electrochemistry


AEB 313