Yaping Chen1,2, Nicolas Voelcker*1,2,3, Roey Elnatnan1,2, Stella Aslanoglou1,2, Babak Nasr4,
Umamageswari Suparamaniam5, Kai Kurashige1

1. Wellington Road Clayton, Moansh University, VIC, Australia
2. 151 Wellington Road Clayton, Melbourne Centre for Nanofabrication, VIC, Australia
3. Research Way Clayton, CSIRO, VIC, Australia
4. Grattan Street Parkville, The University of Melbourne, VIC, Australia
5. John Street, Hawthorn, Swinburne University of Technology, VIC, Australia

The interface between nanotechnology and life sciences is one of the fastest growing and most promising areas of material science. My project focuses on the engineering of vertically aligned silicon nanowire (VA-SiNW) arrays for the development of next-generation biodevices. VASiNWs have been used in intracellular delivery of a variety of biomolecules, including DNAs, RNAs, peptides and proteins, into mammalian cells. However, the interface between VASiNW and different types of cells, especially immune cells, remains elusive. Here, we investigated VA-SiNWs, with different geometrics, for their ability in influencing cellular adhesion, viability, morphology, migration, proliferation, and differentiation during short-term (1 hr) or long-term culture (1 day). It showed that VA-SiNW induced cell attachment while causing minimal toxicity to both adherent fibroblasts and suspension immune cells during short-term (1 hr) culture, whereas suspension immune cells exhibited a significant lower viability than adherent fibroblasts after 1 day culture. Interestingly, it was evident that blunt nanowire (diameter ~300nm) was more suitable for adherent fibroblast adhesion than sharper ones (diameter <100nm). On the contrary, however, suspension immune cells preferred sharper nanowires where they maintained a higher viability. Confocal, SEM and FIB images further confirmed the penetration of nanowires into cell nucleus. In addition, I also assessed the transfection efficiency of plasmid DNAs into immune cells utilising VA-SiNWs. The result has shown that within the 1 hr culture, cells were able to land onto the NWs and uptake coated plasmid DNAs, with its reporter GFP gene expressed 1 day after cell detachment from NWs. This study provides a novel bio-platform for various gene editing and modification, paving the way for the application of a safer and high-throughput nanotechnology into therapeutic interventions in treating numerous diseases and cancers.

Biographic Details

Yaping Chen
Monash University, Australia
Phone: +61 452227190
E-mail: crystal.chen@monash.edu
Research interests: nanowire, nanoneedle, biomaterial,
gene modification, immunotherapy