Kaylene Young*


University of Tasmania,


Experimentally increasing neuronal activity rapidly increases oligodendrogenesis and myelination in the adult brain.  We aimed to determine whether repetitive transcranial magnetic stimulation (rTMS), a non-invasive form of brain stimulation, could produce a similar effect.  To do this we used a 120mT mouse rTMS coil, to deliver a sham stimulation or 600 pulses of rTMS as 10Hz, intermittent theta burst (iTBS) or continuous theta burst pattern.  P90 Pdgfrα-CreERT2 :: Rosa26-YFP and Pdgfrα-CreERT2 :: TaumGFP transgenic mice were used to fluorescently label oligodendrocyte progenitor cells (OPCs) and allow us to trace their adult-born oligodendrocyte progeny.  We determined that 14 days of iTBS treatment significantly increased the number of new oligodendrocytes detected in the motor and visual cortex, and also increased the length of the myelin internodes produced by oligodendrocytes added to the motor and visual cortex, as well as the underlying corpus callosum.  Our data indicate that rTMS can be used to promote oligodendrocyte addition and myelination in the mature brain.     


AEB 301