Julia Walker, 1 Kye Robinson, 1 Christopher Munro, 2 Linda Lua, 2 Simon Corrie1*


1Department of Chemical Engineering, Monash University, Clayton, Victoria, 3800, ARC Centre of Excellence in Convergent BioNano Science and Technology
The University of Queensland, Protein Expression Facility, Brisbane, QLD 4072, Australia


Anti-fouling polymers are of major research interest to reduce protein fouling and foreign body response for implanted medical devices. The most commonly used polymer for its stealth properties is polyethylene glycol (PEG), due to its broad coverage in the literature and the commercial availability of a range of PEG-containing polymers and reagents. However, in order to synthesise biofunctional films from synthetic polymers that incorporate chemical “motifs” for surface immobilization, anti-fouling, and oriented biomolecule attachment, multiple reaction steps are required to be carried out at the solid/liquid interface.

An alternative approach is to express recombinant peptides that contain the required chemical motifs, which arise from the diversity of amino acid side-chain chemistry and monomer-level control afforded by genetic engineering. EK is a non-fouling peptide with alternating units of glutamic acid (E) and lysine (K) which can be recombinantly expressed in bacterial cells. A protein memetic of the EK peptide has been previously reported for resistance to nonspecific protein adsorption due to its strong hydration layer created on the surface from the zwitterionic molecular structures (REF). The EK peptide can be expressed with different chain length and end-groups; in this work two different chain lengths were expressed (5kDa and 12kDa) as well as a 12kDa with a IgG specific Z-domain on end of the peptide. Herein we report our progress with a bacterial expressed EK zwitterionic peptide surface coating of  mixed chain length with selective capture of IgG though an orientated Z-domain on the surface of organo silica nanoparticles. 


1.Yang Q, Wang L, Lin W, Ma G, Yuan J, Chen S, 2014, 2, 577-584, Journal of Materials Chemistry B


Biographic Details

Name: Julia Walker

Title: Ms.

Affiliation, Country: Australia

Phone: +61 478 516 622, E-mail: julia.walker1@monash.edu 

Research interests: biosensors, surface modification.  


Hawken N201