Yuxiao Liu, Yuanjin Zhao*

 

School of Biological Science and Medical Engineering, Southeast University
State Key Laboratory of Bioelectronics
Nanjing,
Jiangsu, China

 

Microcarriers have a demonstrated value for biomedical applications, especially for drug delivery and three-dimensional (3D) cell culture. Attempts to develop this technique tend to focus on the fabrication of specific microparticles both with innovative materials and appropriate methods. Here, we present a simple microfluidic system to prepare egg white (EW) porous microcarriers for 3D cell culture and drug delivery. As one of the most common biomaterials, egg, especially EW, has been used in the biomedical field for centuries. EW is easy to obtain, low cost and has various bioactive functionalities, such as biocompatible, healing-enhancing, antibacterial, cell growth stimulatory properties, and the biodegradability in vivo.1 However, due to the lack of efficient and appropriate preparation methods, the application of EW has still been limited. The microfluidic techniques have vast advantages in controlling the structures of emulsions, they have become a versatile and promising method for preparing monodisperse single or multiple emulsion droplets. Furthermore, novel microcarriers can be created by combining the sonic, optical, heating, magnetic and/or other means with the microfluidics.2 Here, we created a heating microfluidic approach to ‘cook’ sphere microcarriers by using EW, as outlined in Figure 1. After heating the oil bath the EW emulsions were solidified. The resultant microcarriers are highly monodisperse and suitable for cell adherence and growth due to the excellent biocompatibility. And there will be irregular porous structure on the surface of the EW microparticles after freeze-drying, which provide these particles with the ability of absorbing drugs and sustained drug delivering. All these features make the EW porous microparticles described here ideal to be the microcarriers for 3D cell culture and drug delivery.

Figure 1: (a) Schematic of the EW microcarriers generation by using a droplet capillary microfluidics. (b) Schematic of the EW microcarriers for cell culture. (c) Schematic of the freeze-drying egg microcarriers as delivery systems.

References

1 Jalili-Firoozinezhad, S. Rajabi-Zeleti, S. Mohammadi, P. Gaudiello, E. Bonakdar, S. Solati-Hashjin, M. Marsano, A. Aghdami, N. Scherberich, A. Baharvand, H. Martin, I. Adv. Healthcare Mater. 2015, 4, 2281-2290. Facile Fabrication of Egg White Macroporous Sponges for Tissue Regeneration.

2 Shang, L. R. Cheng, Y. Zhao, Y. J. ACS Appl. Mater. Interfaces 2017, DOI: 10.1021/acs.chemrev.6b00848. Emerging Droplet Microfluidics.

 

Biographic Details

Yuxiao Liu

Title: Ms.

Affiliation, Country: State Key Laboratory of Bioelectronics, China

Phone: +86 15651629836 E-mail: yxliu@seu.edu.cn

Research interests: microfluidics, mcirocarriers, drug delivery