Osteogenic Differentiation of Mesenchymal Stem Cells on Aligned Nanofibrous Mats with Bone Mimicking Features
Mohammad Soheilmoghaddam1, Justin Cooper-White1,2,3,*
1 Tissue Engineering and Microfluidics Laboratory, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
2 School of Chemical Engineering, The University of Queensland, Australia 4072
3 Biomedical Manufacturing, Manufacturing Flagship, CSIRO, Clayton, Victoria, Australia,
*(J.J.C.-W.) Tel: +61 3346 3858. E-mail: j.cooperwhite@uq.edu.au.
Assembling biological and structural scaffold that mimic the well-designed architecture of the native bone has become an essential element in bone tissue-engineering. Modular tissue engineering approach for fabricating an ideal extracellular matrix (ECM) with favorable architecture to achieve successful bone integration and regeneration were developed. Here we bottom-up assembled a series of aligned (PLGA) bioactive electrospun fibers reinforced with rod-shape nano-hydroxyapatite (n-HA) to construct osteon-like structures. These aligned nano fibers modified with highly oriented n-HA showed a significant improvement in mechanical properties. Furthermore, the bone-bonding ability of electrospun scaffolds using simulated body fluid (SBF) solution, revealed a substantial enhancement of bonelike apatites of the PLGA/nHA composites. The osteogenic differentiation of human mesenchymal stem cells (hMSCs) was confirmed significantly higher alkaline phosphate activity as well as calcium mineralization of the nanocomposites compared to pure PLGA fibers while the cells orientation followed the underlying nanofiber morphology. Our study demonstrated that tailored aligned nanofibers with favorable mechanical properties in the presence of osteo-conductive n-HA could be a promising candidate for engineering bone tissue.
Biographic Details
Name : Mohammad Soheilmoghaddam
Title: PhD Candidate
Affiliation, Country: Tissue Engineering and Microfluidics Laboratory, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
Phone: +61 444681231 E-mail: Mohammad.Soheilmoghaddam@uq.net.au
Research interests: : Nanocomposites, Biomicrofluidics, Stem Cells, Stem Cells and Tissue Engineering
Name : Justin Cooper-White
Title: Professor
Affiliation, Country: Tissue Engineering and Microfluidics Laboratory, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
Phone: +61 7 334 63858 E-mail: j.cooperwhite@uq.edu.au.
Research interests: Soft Materials, Biomicrofluidics, Stem Cells, Stem Cells and Tissue Engineering