Mai N. Vu1, Song Yang Khor1, Nghia P. Truong1, John F. Quinn1, Michael R. Whittaker1, Thomas P. Davis1*


1ARC Centre of Excellence in Convergent Bio-Nano Science & Technology,
Monash Institute of Pharmaceutical Science, Monash University,
381 Royal Parade, Parkville, Melbourne, VIC 3052, Australia

 

Cellular interactions of nanoparticles with different physiochemical properties have been of great interest. Most of the in vitro experiments performed under static flow condition where cells at the bottom of a culture slide were exposed to a suspension of nanoparticles.1 Cellular uptake of nanoparticles depends on sedimentation and diffusion velocities of the particles.2 However, in in vivo systems, fluidic shear stress that is generated by blood flow in vascular microenvironment or interstitial flow in extracellular matrix may influence interactions between particles and cells.3 Here, synthetic microvascular networks (SMN) provided by SynVivo were used to simulate the dynamic fluid flow in microvasculature.4 The interactions of nanoparticles bearing four different surface groups (carboxyl, tertiary amine, methyl, polyethylene glycol), and three different sizes ( ̴40 nm, ̴ 80 nm, ̴ 130 nm) in the presence of Human Umbilical Vein Endothelial Cells (HUVEC) in the microfluidic system were investigated and compared to equivalent static assays. We found a significant decrease in cellular association of nanoparticles under flow conditions. In both stasis and flow both positive and large particles showed enhanced association in contrast to that of negative, neutral and smaller particles. Another platform of the SMN, modified to incorporate tumour growth areas, was used to implement tumour accumulation studies. Data indicated higher accumulation and association of negative particles when compared to positive counterparts.

 

References

1 Strobl, F. G. et al. Beilstein J. Nanotechnol. 6, 414–419 (2015).

2 Cho, E. C., Zhang, Q. & Xia, Y. Nat. Nanotechnol. 6, 385–91 (2011).

3 Kang, T., Park, C., Choi, J.-S., Cui, J.-H. & Lee, B.-J. J. Drug Deliv. Sci. Technol. 31, 130–136 (2016).

4 Doshi, N. et al. J. Control. Release 146, 196–200 (2010).

 

Biographic Details

Name: Mai N. Vu

Title: Ms

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University, Australia

Phone: 0426 580 930 E-mail: Ngoc.Vu@monash.edu

Research interests: microfluidics, nanopolymers, cellular uptake