Justyna Ciejka*1,2, Krzysztof Pyrć1,3, Krzysztof Szczubiałka2, Maria Nowakowska2

 

1Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University
2Physical Chemistry Department, Faculty of Chemistry, Jagiellonian University
3Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University
Address: The Campus of the 600th Anniversary of the Jagiellonian University Revival, Gronostajowa Street, Krakow, Poland

 

Human coronaviruses are classified as common cold viruses. They cause seasonal upper and lower respiratory tract diseases of varying severity, but sometimes causing epidemics. Undoubtedly, permanent presence of these pathogens in human population and emergence of new viral strains urge the need for effective treatment, diagnostic strategies and removing of viruses from contaminated aqueous environment. All these can be achieved by development of materials specifically interacting with the viral particles. Polymers seem to offer such possibility.

The presentation will highlight the potency of using novel polymeric materials, in the form of both water-soluble polymers and polymeric nano/microspheres, as viral inhibitors. The subject will be discussed according to current studies.

Briefly, we have found that a cationic derivative of chitosan, i.e. N-(2-hydroxypropyl)-3-trimethyl chitosan (HTCC), shows strong anticoronaviral activity.1 The specificity of its antiviral properties is high and is correlated with the degree of cationization. Further mechanistic studies indicated that HTCC interacts with the viral particle and may be used as a broad-range inhibitor of human coronavirus entry.2

In order to extend the spectrum of application of HTCC, nano/microspheres were obtained from chitosan and cross-linked with genipin. Their surface was modified with glycidyltrimethylammonium chloride, then used as specific coronavirus adsorbents.3 The aim of these studies includes the development of effective methods of removal, purification and/or concentration of the dispersion of coronaviruses.

References:

1 Milewska A., Ciejka J., Kaminski K., Karewicz A., Bielska D., Zeglen S., Karolak W., Nowakowska M., Potempa J., Bosch B.J., Pyrc K., Szczubialka K., Antiviral Research 2013, 97, 112-121. Novel polymeric inibitors of HCoV-NL63

2 Milewska A., Kaminski K., Ciejka J., Kosowicz K., Zeglen S., Wojarski J., Nowakowska M., Szczubialka K., Pyrc K., Plos One 2016, 11 (6), art. no. 0156552. HTCC: broad range inhibitor of coronavirus entry.

3 Ciejka J., Wolski K., Nowakowska M., Pyrc K., Szczubialka K., Materials Science and Engineering C 2017, 76, 735-742. Biopolymeric nano/microspheres for selective and reversible adsorption of coronaviruses

 

Acknowledgements:

National Science Center, Poland, provided funding to Justyna Ciejka under grant number UMO-2016/21/D/ST5/01661

 

Biographic Details

Name: Justyna Ciejka

Title: Dr

Affiliation, Country: Malopolska Centre of Biotechnology, Jagiellonian University; Faculty of Chemistry, Jagiellonian University; Krakow, Poland

Phone: +48 12 664 79 55 Fax: +48 12 664 6902 E-mail: justyna.ciejka@uj.edu.pl

Research interests: polymer chemistry, biopolymers, adsorbents, viruses, antiviral drugs, adsorbents of human viruses