Kostya (Ken) Ostrikov*, Kateryna Bazaka

 

Institute of Health and Biomedical Innovation
Queensland University of Technology
Brisbane, QLD, Australia
e-mail: Kostya.Ostrikov@qut.edu.au

 

The presentation will highlight two examples of interfacing low-temperature plasmas with objects of relevance to bio-sensing and treatment of cancer and other diseases such as neurodegeneration. The first example focuses on ultra-sensitive geno-sensors for early disease onset detection and nano-carries for intracellular gene delivery. The second example is related to direct agitation of cancer, stem and other cells by plasmas, which can be implemented in animal models of human diseases. 

In the first example, Al-oxide nanoparticles are coated with a carbon nano-layer using plasmas. The structure is multi-functional, soluble in water, bio-compatible and its surface chemistry can be tuned. These particles are used for cell monitoring and gene delivery at the same time, and they carry nucleic acids into the cells and then release. The nanoparticles were demonstrated in successful intracellular delivery, including internalization and endosomal escape. Fluorescence from the core of the particles helps monitor them in living organisms. These nanoparticles are promising as nano-carriers for targeted gene and drug delivery, with simultaneous intracellular activity monitoring.

The second example presents atmospheric-pressure plasmas as promising cancer therapy. It can selectively kill cancer cells through apoptosis. In vitro results show that reactive oxygen species (ROS) trigger apoptosis-related intracellular signaling cascades. Mechanisms include oxidative stress and interference with DNA, proteins, and other cellular components. Our study supports the ROS-mediated mechanism of cancer cell death. To compare the mechanisms and evaluate the correlation between in vitro and in vivo outcomes, Mel-007 cells were injected subcutaneously into mice to form solid tumors. The tumors were then treated with AP and the results of different treatments compared. Challenges associated with translating the in vitro results into corresponding in vivo outcomes are discussed and the applicability of mechanisms established in vitro during the translation to dynamic in vivo environments is discussed. Other interesting examples of effective applications of atmospheric pressure plasmas for the effective control of intracellular responses are also presented. 

Biographic Details

Name: Kostya (Ken) Ostrikov

Title: Professor

Affiliation, Country: Queensland University of Technology

Phone: +61 7 3138 7659 E-mail: kostya.ostrikov@qut.edu.au

Research interests: atmospheric pressure plasmas, nanomedicine, nanofabrication, graphene