Matthew Henry, Dr. Marianne Gillard, Michael Yeh, Prof. Stephen Mahler*, Prof. Peter Gray

 

Building 75, Corner of College Road and Cooper Road, UQ,
St Lucia, 4072
AIBN
Brisbane, QLD, Australia

 

 A screening platform to evaluate whether novel genes of interest, (identified from a SWATH comparative proteomics dataset1) increase antibody yield in Chinese hamster ovary cells that stably express a monoclonal antibody (mAb), is presented. Although there has been recent progress in the understanding of mAb production processes in CHO cells, 2 3, there is a need for further understanding of CHO cell biology so that this industrially relevant cell line can be engineered and optimised for the production of next generation antibodies utilising new bioreactor formats (e.g. perfusion). It is envisaged that data generated from these experiments will support some of the rational engineering strategies of the future, enabled by advanced technologies such as CRISPR-Cas9 4. 

1.     Orellana CA, Marcellin E, Schulz BL, Nouwens AS, Gray PP, Nielsen LK. High-Antibody-Producing Chinese Hamster Ovary Cells Up-Regulate Intracellular Protein Transport and Glutathione Synthesis. J Proteome Res. 2014;14:609-618. doi:10.1021/pr501027c.
2.     Fischer S, Handrick R, Otte K. The art of CHO cell engineering: A comprehensive retrospect and future perspectives. Biotechnol Adv. 2015;33(8):1878-1896. doi:10.1016/j.biotechadv.2015.10.015.
3.     Hansen HG, Pristovšek N, Kildegaard HF, Lee GM. Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions. Biotechnol Adv. 2016. doi:10.1016/j.biotechadv.2016.11.008.
4.     Lee JS, Grav LM, Lewis NE, Kildegaard HF. CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives. Biotechnol J. 2015;10(7):979-994. doi:10.1002/biot.201500082.

Biographic Details
Name: Matthew Neil Henry
Title: Mr
Affiliation, Country: AIBN, Australia
Phone: (+61) 0487 666 145 E-mail: matthew.henry2@uqconnect.edu.au
Research interests: Secretory Pathway, Antibodies, CHO Cell Engineering
 

Venue

Room: 
AEB 301