Rebecca Wood, Kaori Yoneyama, Manuel Plan, Koichi Yoneyama, Christine Beveridge, Claudia Vickers*

 

Australian Institute for Bioengineering and Nanotechnology (AIBN)
The University of Queensland
Brisbane, Queensland
Australia

 

Strigolactones are a chemically diverse group of plant hormones with strong potential in agriculture for controlling plant parasites and enhancing crop productivity, thereby contributing to improved food security. However, agricultural applications are impeded by the prohibitively expensive chemical synthesis, and a poor understanding of how diverse strigolactones differentially contribute to pleiotropic hormonal functions. A microbial production platform may offer an economical route to strigolactone synthesis, and provide a platform to investigate the biosynthesis and physiological activities of diverse strigolactones. Here, we report the first steps in metabolic engineering of Saccharomyces cerevisiae for strigolactone production. β-carotene production was engineered using genes from the carotenogenic yeast Xanthophyllomyces dendrorhous. Expression of the SL biosynthesis enzymes D27, CCD7, and CCD8 from Arabidopsis thaliana converted β-carotene to the SL precursor carlactone. The resulting low-titre, proof-of-principal strain will be the basis for further engineering for yield enhancement and the production of diverse strigolactones.

 

Biographic Details

Name: Rebecca Wood

Title: Ms

Affiliation, Country: University of Queensland, Australia

Phone: +61733463489

E-mail: r.wood@uqconnect.edu.au

Research interests: synthetic biology, strigolactones, phytohormones yeast, metabolic engineering